Abstract:
The development of resistance in pathogens to existing drugs is one of the world's biggest health problems. In this study, in order to contribute to new antibacterial drug research, two fluorophenyl-butylsalicysyl aldimine Schiff base ligands and their antibacterial activities on Klebsiella pneumoniae, Proteus mirabilis and Streptococcus pyogenes containing copper and palladium complexes were investigated by MTT analysis. Chemicals and positive control (gentamicin) were seeded on microplates in triple order as 1μM, 10μM, 100μM and 1000μM, and bacterial strains were added at a dose of 0.5 McFarland. After 18 h incubation, dimethyl sulfoxide was used as a solvent to dissolve formazan crystals after applying MTT dye as a viability indicator to determine the MIC50 values of each of the compounds. OD values obtained with a microplate reader at 570 nm wavelength for each well were used to calculate the MIC50 of each chemical. Gentamicin MIC50 values were determined to be 2.09, 1.87, and 1.68 µM for K. pneumoniae, P. mirabilis, and S. pyogenes, respectively, and the strongest MIC50 values against K. pneumoniae and P. mirabilis were by C1 and C4, respectively. It was determined as 6.06 and 5.31 µM. While S. pyogenes exhibited strong activity against almost all compounds, the most effective MIC50 value was determined as 3.72 µM with C3. In our study, we think that the strong antibacterial effect of Schiff base copper complex on S. pyogenes is particularly noteworthy.
