Timokinin'in vasküler endotel hücrelerinde iskemi/reperfüzyon hasarının önlenmesinde etkinliğinin araştırılması / Investigation of efficiency of timokin on vascular endothelial cells' ischemia / reperfusion injury

Abstract

GiriĢ: ÇeĢitli çalıĢmalarda akciğer, böbrek ve karaciğer gibi organlarda Timokinin'in (TQ) iskemi reperfüzyona karĢı koruyucu etkisi gösterilmiĢtir. Biz Timokinin'in abdominal aortada iskemi reperfüzyon hasarında vasküler doku üzerine olası etkisi araĢtırılması amaçlandı. Materyal Metod: OnbeĢ sıçan sham (n:5), kontrol(n:5) ve TQ-tedavi grubu(n:5) olarak üçe ayrıldı. Kontrol ve tedavi grubuna 60 dakika abdominal aort iskemi sonrası 120 dakika reperfüzyon uygulandı. Tedavi grubunda TQ reperfüzyonndan 5 dakika önce 200 mg/kg intraperitoneal verildi. Kan serumunda Total antioksidan kapasite (TAS), Total oksidatif stres (TOS), Oksidatif stres indexi (OSI) ölçüldü, aort dokusu histopatolojik olarak ıĢık mikroskopunda incelendi. Sonuçlar: TOS ve OSI aktivitesi sham ve tedavi grubunda, kontrol grubuna göre anlamlı olarak düĢük idi (p <0.001 for TOSand OSI). Kontrol grubuna göre tedavi grubu hasar skoru eĢit idi. TartıĢma: Akut abdominal aort iskemi reperfüzyon sıçan modelinde Timokinin'in intraperitoneal verilmesi oksidatif stresin azalmasında etkilidir fakat aort dokusu histopatolojik olarak iskemi reperfüzyondan etkilenmemiĢtir. Introduction:Previous studies have demonstrated that thymoquinone (TQ) has protective effects against ischemia reperfusion injury to various organs like lung, renal, hepatic in different experimental models. We aimed to determine whether thymoquinone has favorable effects on vascular tissues and oxidative stress in abdominal aorta ischemia- reperfusion injury. Material and methods:Fifteen rats were divided into three groups as sham (n:5), control (n:5) and TQ treatment group (n:5). Control and TQ-treatment groups underwent abdominal aorta ischemia for 60 min followed by a 120 min period of reperfusion. In the TQ-treatment group, TQ was given 5 min. before reperfusion at a dose of 20 mg/kg via an intraperitoneal route. Total antioxidant capacity (TAC), total oxidative status (TOS), and oxidative stress index (OSI) in blood serum were measured, and aort tissue histopathology were evaluated with light microscopy. Results:TOS and OSI activity in blood samples were statistically increased in the control group compared to the sham and TQ-treatment groups (p <0.001 for TOS and OSI). Control group injury scores were statistically equal compared to sham and TQ-treatment groups (p <0.001 for all comparisons). Conclusions:TQ administered intraperitoneally was effective in reducing oxidative stress in an acute abdominal aorta I/R rat model but histopathologically aorta was not affected by the injury.