DEVELOPMENT OF A DNA ISOLATION PROTOCOL FOR BREAST CANCER TISSUE SAMPLE OF FORMALIN FIXED PARAFFIN EMBEDDED

Abstract

Nucleic acid extraction and utilization methods from formalin-fixed and paraffin-embedded (FFPE) tissues—which are stored in libraries for extended periods of time—have been developed. Nonetheless, DNA taken from FFPE tissues is typically harmed, and DNA quality may be impacted by prolonged storage. Thus, it's critical to understand how long-term preservation affects FFPE tissues and to advance the methods for obtaining sufficient and high-quality DNA from them. In this project, we aimed to create a DNA isolation protocol from FFPE blocks of breast cancer tissues. A total of 30 FFPE blocks of breast cancer tissues from different types of breast cancer had been studied as three replicates. The blocks had been surgically resected and fixed at Harran University Hospital prior to examination and subsequent storage at 4-degree temperature for several years. The average spectrophotometer measurements of DNA quality were 1.9 (A260/A280) and 1.4 (A260/A230). The average DNA amount was 78.2 nano gram per microliter. The results of DNA extraction are suitable for all DNA based methods such as next generation sequencing technologies. The developed DNA extraction protocol has six easy steps with commonly available chemicals and solutions. It is possible to extract DNA from 30 samples less than three hours and it costs 1 US dollar per sample by developed protocol from FFPE tissue blocks of breast cancer. The developed protocol has many advantages to open the treasure box of FFPE blocks in pathology laboratories and libraries.