THE INVESTIGATION OF ANTICANCER EFFECT OF SCORPION CARAPACE

Abstract

Scorpion is one of the arachnids which have several properties, there are many studies on the effectiveness of scorpion venom, despite the danger of poison to humans, studies found that there are many active peptides in scorpion venom, and they are a rich source of pharmacologically active compounds that have a role in helping to cure of infection, and cancer treatment ... etc. From there we developed the study and focused on other aspects of the treatment through Scorpion. The aim of this study is to determine the anti-cancer properties of the carapace of black scorpion (Androctonus crassicauda) that lives in Sanliurfa. This study was determined as the first study done on scorpion carapace to detect the anti-cancer activity .The scorpion's outer shell was collected and extractions of (hexane, dichloromethane, ethanol, methanol, and water) were extracted then the detection of the cytotoxic effect of scorpion shield was done on colon cancer (HCT-116), breast cancer (MDA-MB-231), prostate cancer (DU-145), lung cancer (A-549), bone cancer (U2OS), normal lung (BEAS-2B), kidney (HEK-293), normal prostate (PNT-1A), normal breast (CRL 4010), and endothelial cell (HUVEC) Obtained from ATCC by investigate them by MTT assay .The effect of the strongly cytotoxic fraction on cell apoptosis was investigated by Annexin-V and cell cycle flow cytometry. As a result of the study, it was determined that the hexane fraction showed the strongest cytotoxic effect on prostate cancer cells (DU-145), while its toxic effect on normal prostate cells (PNT-1A) was found ix to be lower than on cancer cells. It was determined that the hexane fraction induced apoptosis on DU-145 and stopped the cell cycle in Sub G1 phase. According to the data obtained, it was concluded that the scorpion carapace has anticancer potential, therefore, further studies should be carried out to determine the components showing anticancer activity.