DSpace Community: Sağlık Bilimleri Fakültesi'ne ait bölümleri içerir

New prognostic biomarker in cardiovascular field: ST2 (IL1RL1)

Tue, 01 Jan 2019 00:00:00 GMT

Title: New prognostic biomarker in cardiovascular field: ST2 (IL1RL1) Authors: Dikme, Reşat; Padak, Mahmut; Göz, Mustafa; Aydın, Mehmet Salih; Göç, Ömer Abstract: As a result of natural immunity in the heart ST2 (IL1RL1) that protects the heart against excessive pressure load and tension, has two important isoforms (ST2L–membranebound, sST2-soluble). As ligand of ST2L and sST2 is interleukin 33 (IL33), the connection of ST2L to IL33 stimulates cardioprotective signal cascade whereas connection of sST2 to IL33 causes annihilation of these positive effects. As a high level of sST2 shows that the heart is under dense stress, this situation causes cell death, tissue fibrosis, decreased cardiac functions and an increase in progression rate of disease. That is why in cardiovascular disease sST2 is accepted as a biomarker of poor prognosis. It was obtained that the increase of sST2 over normal concentration multiplies negative situations related to cardiovascular diseases 3 times more. In 2013 in ACCF/AHA (American College of Cardiology Foundation/American Heart Association) guidelines, sST2 that was defined as “novel biomarker” for HF (Heart failure), is an independent biomarker from natriuretic peptides and cardiac troponins and is an important sign in cardiovascular diseases. ST2 is not affected by factors such as age, body mass index and kidney function disorder.

Liquid reflecting the heart function: Pericardial fluid

Tue, 01 Jan 2019 00:00:00 GMT

Title: Liquid reflecting the heart function: Pericardial fluid Authors: Dikme, Reşat; Padak, Mahmut; Göz, Mustafa; Aydın, Mehmet Salih; Göç, Ömer Abstract: Pericardium is a sac surrounding the heart and big vessels, protecting them against injury and infection and fixing the heart to the mediastinum. As pericardium has an immunologic, paracrine, vasomotor and fibrinolytic activity; it affects myocyte structure, function and gene expression by synthesizing materials such as eicosanoids and prostaglandin. Pericardial fluid (PF) in two-walled pericardial sac surrounding the heart completely, has a potential of reflecting heart function and shows dynamic changes. Since many biomarkers associated with cardiovascular diseases pass to pericardial sac, PF analysis provides to be understood many pathophysiological mechanisms in various pericardial and cardiovascular diseases. The aim of this article is to give actual information about the content of PF that is thought to be passive ultrafiltrate of plasma produced with hydrostatic pressure difference and osmotic concentration gradient for years.

MICRORNAS IN THE PERICARDIAL FLUID OF CORONARY ARTERY PATIENTS

Mon, 01 Jan 2018 00:00:00 GMT

Title: MICRORNAS IN THE PERICARDIAL FLUID OF CORONARY ARTERY PATIENTS Authors: Dikme, Reşat; Gönel, Ataman; Koyuncu, İsmail; Akkafa, Feridun; Göz, Mustafa; Aydın, Mehmet Salih; Temiz, Ebru Abstract: Background: microRNAs (miRNAs) that play important roles in organisms are associated with usual function of cells, development of living, biological processes as well as many diseases involving cardiovascular disease. miRNAs are effective in endothelium disfunction, atherosclerosis, inflammation, apoptosis and angiogenesis related with coronary artery disease (CAD). miRNAs that are explained differently in cells, exist extracellularly, in circulation and body fluids. Many biomarkers related with CAD can get through circulation and pericard fluid (PF), the closest place to heart. That is why in etiopathogenesis evaluation of CAD miRNAs can be used diagnostically in PF and circulation. Since PF has a potential of reflecting heart potential, PF concentration of some substances provides to be understood many pathophysiological mechanisms.

Oxidative stress and DNA damage during cardiopulmonary bypass

Tue, 01 Jan 2019 00:00:00 GMT

Title: Oxidative stress and DNA damage during cardiopulmonary bypass Authors: Dikme, Reşat; Göz, Mustafa; Aydın, Mehmet Salih; Çelik, Hakim; Padak, Mahmut; Göç, Ömer; Taşkın, Abdullah Abstract: Aim: The aim of this study is to measure the oxidative stress index (OSI) and DNA damage in patients undergoing cardiopulmonary bypass (CPB) surgery due to coronary artery disease. Material and Method: 25 patients operated for CPB surgery due to coronary artery disease were included in the study. By collecting heparin containing blood from the patients at 5 different times (Before CPB, during pump inlet, during placing of cross clamp, during taking out of cross clamp and after CPB) DNA damage was studied by Comet Assay method from mononuclear leucocytes and OSI in plasma was studied. Results: At 5 different times plasma OSI value was found respectively as 4.92 ± 1.73, 5.20 ± 2.69, 5.70 ± 2.06, 9.88 ± 4.88, 9.57 ± 4.54 whereas Arbitrary unit (AU), DNA damage were determined respectively as 28.48 ± 11.30, 29.04 ± 9.43, 31.44 ± 11.74, 33.92 ± 7.96, 38.56 ± 6.20 AU and p<0.001. Discussion: During CPB, OSI and DNA damage gradually increased and a positive meaningful correlation occured between these two parameters (r=0,882 p=0,048). As CPB period extended, oxidative stress and DNA damage increased as well as TOS and OSI increased, or vice versa.